Used HPLC column Tosoh TSKgel Boronate-5PW 7.5mm x 7.5 cm 10 um 13066
Used HPLC column Tosoh TSKgel Boronate-5PW 7.5mm x 7.5 cm 10 um, cat no 13066 Lot gar4thmix
TSKgel Boronate-5PW is based on a 100 nm pore size polymethacrylate base material bonded with m-aminophenyl boronate.This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, glycosylated proteins, and catecholamines.
Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ions and is inhibited by amine-containing buffers.Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers, such as HEPES and morpholine, while desorption takes place in carbohydrate or amine-containing mobile phaselike sorbitol or Tris.Applications include nucleic acids, catecholamines, nucleosides, and nucleotides.